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WORD: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and also among the amino groups within the spot areas do not type bonds with the amino acids. OBSERVE: This avoids the coupling of amino acids within the further cycles with the membrane instead of the rising peptide chain. Make sure that all of the purification buffers are at four °C and carry out the protein purification within the chilly room to avoid protein denaturation.
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